NB4-CAS9 cell line

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NB4-CAS9 Order

NB4-CAS9 cell line
Catalog#
YC-C122-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NB4-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
NB4-CAS9
Cell Morphology
Monocyte, suspension
Passage ratio
1：2-1：4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the NB4 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NB4-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
NB4-CAS9 Cas9 21.21349144 8.57135201
NB4-CAS9 β-actin 12.64213943
NB4 Cas9 36.31499863 22.49466324
NB4 β-actin 13.82033539
Cutting Efficiency Validation
NB4-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the NB4-CAS9 stable cell pool which is electroporated by TLN1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NB4		 STR Info (Cell bank)
Cell Line:NB-4
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 101314.01013
D16S539 9999
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 811811
CSF1PO 11121112
D12S391 1922
FGA 202122.0
D2S1338 202325.0
D21S11 2833.2
D18S51 1214
D8S1179 1014
D3S1358 1517
D6S1043 1212
PENTAE 713
D19S433 1515
PENTAD 1013
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
NB4-CAS9 cell line
NB4-CAS9
Catalog#
YC-C122-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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